Screening Assay – Part KIT2021 (D14642964)
for stx and eae
96 PCR tubes with tablets (2 bags of 6 x 8 strips)
96 flat optical caps (12 x 8 strips)
1 bottle of protease (400 µL)
2 bottles of lysis buffer (12 mL)
Food processors and associated laboratories can use the BAX® System STEC suite as a quick and reliable method for detecting pathogenic Shiga toxin-producing E. coli (STEC) in a variety of foods, hemp and cannabis flowers. Each real-time PCR assay in the STEC suite is designed to report clear yes/no results at concentrations as low as 104 cfu/mL after enrichment. The screening assay detects the STEC virulence genes (stx1, stx2 and eae) with a simple, single-stage enrichment; two panel assays can then use the same lysate to determine if an STEC-screening positive is
also positive for one of the top six STEC serogroups (E. coli O26, O45, O103, O111, O121, and O145). With a processing time of approximately 55 minutes in the BAX® System Q7 instrument, the method returns results comparable to culture methods, but with a significantly faster time to result.