Megazyme™ Lactose/Galactose Assay Kit
An enzymatic method for the quantitative determination of lactose and D-galactose in food and biological samples. Suitable for the analysis of low-lactose and lactose-free products, offering high specificity and rapid analysis times.
Description
The Megazyme™ Lactose/Galactose Assay Kit (Rapid) is designed for the specific and rapid analysis of lactose and D-galactose in food, plant material, and biological samples. The method is based on the enzymatic hydrolysis of lactose followed by the quantification of galactose via an NAD⁺-dependent reaction, in which the formation of NADH is measured spectrophotometrically.
The kit is particularly suitable for analyzing products with low lactose content or lactose-free formulations, where high levels of monosaccharides might otherwise interfere with the analysis. The method is well-established and is used in internationally validated standards, including AOAC .06.
Features
- Rapid analysis with high specificity for lactose and D-galactose
- Suitable for low-lactose and lactose-free products
- Enzymatic method with high reproducibility
- Complete kit with standard included
- Stable reagent kit (lasts up to several years when stored properly)
More information
| Parameter | Lactose / D-galactose |
|---|---|
| Result type | Quantitative |
| Analysis time | about 20–30 minutes |
| Number | |
| Storage temperature | 4 °C (component-dependent) |
| Sustainability | Up to several years, depending on the reagent |
| Other specifications | Method principle: Enzymatic (β-galactosidase + galactose dehydrogenase) |
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