Megazyme™ D-Fructose/D-Glucose Assay Kit
The Megazyme™ D-Fructose/D-Glucose Assay Kit is an enzymatic UV assay for the specific determination of D-fructose and D-glucose in plant and food samples. The kit allows for both separate and simultaneous determination of D-glucose and D-fructose in the same sample.
Description
The Megazyme™ D-Fructose/D-Glucose Assay Kit is an enzymatic UV assay for the quantitative determination of D-fructose and D-glucose in food and plant-based samples. The method is based on enzymatic phosphorylation followed by NADPH formation, with absorbance measured at 340 nm. D-glucose is quantified directly, while D-fructose is determined after enzymatic conversion to glucose-6-phosphate. This allows for both separate and sequential determination of each sugar type in the same sample.
The kit includes 110 assays using a manual method and up to 1,100 assays using microplate or automated systems.
Features
- Specific quantification of both D-fructose and D-glucose.
- Suitable for food and beverage samples such as wine, beer, and juice.
- Quick analysis (~13 min)
- AOAC method
Instructional video
More information
| Parameter | D-Fructose/D-Glucose |
|---|---|
| Result type | Quantitative |
| Analysis time | ~13 min. |
| Application | Enzymatic determination of D-fructose and/or D-glucose in plant and food products |
| Number | |
| Storage temperature | 4 °C and -10 °C, depending on the reagent |
| Sustainability | > 2 years under recommended storage conditions |
| Other specifications | Method type: Enzymatic UV method |
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