Megazyme™ Total Dietary Fiber Assay Kit
The Megazyme™ Total Dietary Fiber Assay Kit is an enzymatic-gravimetric assay kit for the determination of total, soluble, and insoluble dietary fiber in accordance with established AOAC.
Description
The Megazyme™ Total Dietary Fiber Assay Kit is an enzymatic-gravimetric assay kit for the determination of dietary fiber in accordance with AOAC .43 and 985.29. The kit includes two method protocols: one for the determination of total, soluble, and insoluble dietary fiber, and an alternative protocol for total dietary fiber only.
The method is based on the enzymatic breakdown of starch and protein, followed by gravimetric quantification of the fiber fraction. The kit is primarily used for routine analysis of food and raw materials and does not require HPLC.
Features:
- AOAC .43 / 985.29 validated method
- Two assay protocols in the same kit
- Measures total, soluble, and insoluble dietary fiber
- No HPLC required
How to use the product:
Method 1
Method 2
More information
Instructions
Instructions (PDF, 253 KB)
Calculation template (PDF, 806 kB)
Product sheets and guides
Dietary Fiber Analysis Guide (PDF, 386 KB)
Industries Overview Flyer (PDF, 4MB)
Food and Beverages Brochure (PDF, 2MB)
Enzymatic Assay Kit Workflow Flyer (PDF, 143 KB)
Megazyme(PDF, 494 KB)
| Parameter | Dietary fiber (total, soluble, insoluble) |
|---|---|
| Result type | Quantitative |
| Analysis time | approx. 16–20 hours (including overnight incubation) |
| Application | Food, raw materials, animal feed |
| Number | |
| Storage temperature | 2–8 °C (enzymes); other components as indicated on the label |
| Sustainability | See the expiration date on the package (depends on the component) |
| Other specifications | Enzymatic-gravimetric method using α-amylase, protease, and amyloglucosidase. Alcohol precipitation and gravimetric determination. |
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