Megazyme™ Integrated Total Dietary Fiber Assay Kit
Enzymatic test kit for the quantitative determination of total dietary fiber in accordance with AOAC .01 and 2011.25. Covers both high- and low-molecular-weight fiber fractions, including resistant starch and oligosaccharides.
Description
The Megazyme™ Integrated Total Dietary Fiber Assay Kit is an enzymatic assay kit for the quantification of total dietary fiber in foods and raw materials in accordance with AOAC .01 and 2011.25. The method covers both high- and low-molecular-weight fiber fractions, including resistant starch and indigestible oligosaccharides, in accordance with the CODEX definition of dietary fiber. The assay combines enzymatic digestion with gravimetric determination and HPLC, providing a complete and accurate fiber profile.
This is a reference method for the analysis of total dietary fiber when high accuracy and a complete fiber profile are required.
Features:
- Reference method according to AOAC CODEX
- Measures both high- and low-molecular-weight dietary fiber
- Includes resistant starch and oligosaccharides
- Provides more comprehensive results than older methods
- Suitable for complex food and regulatory analyses
How to use the product:

More information
Instructions
Instructions (PDF, 253 KB)
Calculation template (PDF, 806 KB)
Product sheets and guides
Dietary Fiber Analysis Guide (PDF, 386 KB)
Quality Control and Nutritional Analysis – Brochure (PDF, 4MB)
Quality Control and Nutritional Analysis – Product Sheet (PDF, 494 KB)
Food and Beverage – Brochure (PDF, 2MB)
Enzymatic Analysis Kits – Product Sheet (PDF, 143 KB)
| Parameter | Total dietary fiber (TDF) |
|---|---|
| Result type | Quantitative |
| Analysis time | ~3 hours of work (over 2 days) |
| Application | Food, ingredients, and other materials |
| Number | |
| Storage temperature | 2–8°C |
| Sustainability | At least 24 months (lot-specific) |
| Other specifications | Method type: Enzymatic |
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