Megazyme™ Rapid Integrated Total Dietary Fiber Assay Kit

The Megazyme™ Rapid Integrated Total Dietary Fiber Assay Kit is intended for the quantitative analysis of total dietary fiber in food. The method is based on an integrated enzymatic process in which starch and protein are broken down under controlled conditions that simulate digestion in the small intestine.

The analysis includes an extended incubation period (4 hours), which enables accurate quantification of resistant starch and non-degradable carbohydrates. This is particularly important when analyzing modern food products, where traditional methods may yield inaccurate results.

The method has been developed to comply with current definitions of dietary fiber and minimizes the formation of analytical artifacts compared to older AOAC.

The kit has been validated in accordance with AOAC .16, AOAC .01, AACC 32-60.01, and ICC 185.

Features

• Integrated enzymatic analysis of total dietary fiber
• Simulates digestion in the small intestine (4-hour incubation)
• Accurate quantification of resistant starch (including RS4)
• Minimizes artifacts from older AOAC methods
• Designed for modern food matrices
• AOAC .16 and 2022.01 validated method