foodproof® Beer Screening 2 LyoKit
PCR for the detection of beer-spoiling bacteria in beer and during the brewing process using variant-controlled multiplexPCR.
Description
foodproof® Beer Screening 2 LyoKit is aPCR for rapid screening of beer-spoiling bacteria in beer, yeast, and enrichment samples. The assay is strain-specific and is based on multiplex detection of both lactic acid bacteria and strict anaerobic bacteria, as well as genes associated with hop tolerance.
The method enables the simultaneous detection of Lactobacillus and Pediococcus, as well as Pectinatus and Megasphaera, in separate detection channels, along with horA/horC genes that indicate the ability to grow in beer. This provides a functional decision-making tool rather than merely species detection.
The kit contains pre-lyophilized reagents, an internal control, and an UNG system to minimize the risk of contamination. The workflow is optimized for routine analysis in breweries, offering high reproducibility and minimal hands-on time.
Features
- MultiplexPCR the simultaneous detection of multiple bacterial groups
- Variant-driven analysis (LP, RP, DP depending on the instrument platform)
- Detection of >30 beer-spoiling bacteria
- Includes hop tolerance genes (horA, horC)
- Internal control for the validation of negative results
- Freeze-dried reagents for reliable handling
- High sensitivity after enrichment (down to low CFU levels)
Instructional video:

More information
| Parameter | Beer-spoiling bacteria |
|---|---|
| Test type | Real-timePCR |
| Analysis time | about 2–3 hours |
| Number | |
| Storage temperature | 2–8°C |
| Sustainability | Batch-dependent, as per packaging |
| Assay variants | KIT230074. LP = Lactobacillus spp. + Pediococcus spp., KIT230075. RP = Pectinatus spp. + Megasphaera spp., KIT230076. DP = Multiplex panel (all of the above + horA/horC) |
| Other specifications | Detection channels: FAM, HEX, ROX, (Cy5/ATTO depending on |
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