Helica® Aflatoxin B1 (Low Matrix) ELISA
Quantitative ELISA the determination of aflatoxin B1 in raw materials with a high matrix effect, such as spices, silage, oils, nuts, and grains. The method is designed for use in a laboratory setting and provides results in approximately 70 minutes with a reading at 450 nm.
Description
Helica® Aflatoxin B1 Low Matrix ELISA a quantitative immunoassay kit for the analysis of aflatoxin B1 in complex matrices where standard methods are often affected by matrix effects. The product is intended for quality control and laboratory analysis of, among other things, grains, nuts, cottonseed, feed, and other raw materials that are difficult to analyze.
The method is based on competitive ELISA, in which aflatoxin B1 in the sample competes with an enzyme-labeled conjugate for binding to antibody-coated microwells. After incubation, washing, and a substrate reaction, the absorbance is measured at 450 nm, and the result is plotted against a standard curve.
The kit is designed for matrices whereELISA a clear advantage, such as spices, silage, vegetable oils, and infant formula. Hygiena also Hygiena good recovery in several challenging matrices, as well as good correlation with HPLC in chili samples.
Features
- Quantitative ELISA aflatoxin B1
- Designed for complex matrices with high matrix effects
- Reading time: approximately 70 minutes
- 96 wells with break-away strips
- Measurement in a microplate reader at 450 nm
More information
| Parameter | Aflatoxin B1 |
|---|---|
| Test type | ELISA |
| Result type | Quantitative |
| Analysis time | about 70 minutes |
| Number | |
| Storage temperature | 2–8°C |
| Sustainability | Batch-dependent, as per packaging |
| Other specifications | Absorbance at 450 nm |
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