Megazyme™ Ammonia Assay Kit (Rapid)

The Megazyme™ Ammonia Assay Kit (Rapid) is designed for the rapid and specific analysis of ammonia in a wide range of matrices, including food, beverages, and biological samples. The method is based on an enzymatic reaction in which ammonia is converted in the presence of glutamate dehydrogenase, resulting in a measurable change in absorbance at 340 nm.

The assay is optimized for high reproducibility and fast reaction times (~5 minutes), making it suitable for both routine testing and process control. The kit can be used in multiple assay formats, including spectrophotometer, microplate, and automated analyzer, offering flexibility depending on the laboratory’s equipment.

This method is particularly relevant in wine and food analysis, where ammonia (including YAN—Yeast Available Nitrogen) is a key parameter for quality control and fermentation monitoring.

Features

• Quick analysis time (~5 min)
• High specificity via glutamate dehydrogenase
• Measurement at 340 nm (NADPH)
• Wide range of applications (food, beverages, biological samples)
• Flexible format: cuvette, microplate, or autoanalyzer
• Stable reagents (>2 years)
• Low detection limit (≈0.07 mg/L)