Megazyme™ α-Amylase Assay Kit (Ceralpha Method)

The Megazyme™ α-Amylase Assay Kit (Ceralpha Method) is designed for the quantitative determination of α-amylase in, for example, cereal flour, cereal samples, and fermentation broths. The method uses a defined oligosaccharide substrate, blocked p-nitrophenyl maltoheptaoside, together with thermostable α-glucosidase. When the substrate is hydrolyzed by α-amylase, free reaction products are formed that can be measured spectrophotometrically at 400 nm.

The kit is intended for laboratories that wish to measure α-amylase with high method-specificity and in accordance with well-established standard methods. Megazyme specifies the product Megazyme suitable for the analysis of cereal samples and fermentation broths, and the method is recognized under, among others, AOAC .01, AACC 22-02.01, and ICC Standard No. 303. The total analysis time is stated as approximately 30 minutes.

Features

• Specific method for determining α-amylase.
• Spectrophotometric measurement at 400 nm.
• An established method recognized by AOAC, AACC, and ICC.
• Suitable for grain samples and fermentation broths.