Megazyme™ Catalase Assay Kit
The Megazyme™ Catalase Assay Kit is intended for the quantitative determination of catalase activity in food, biological, and microbiological samples. The method is based on the decomposition of hydrogen peroxide and subsequent colorimetric detection at 520 nm.
Description
The Megazyme™ Catalase Assay Kit is a colorimetric method for analyzing catalase activity based on the enzyme’s ability to break down hydrogen peroxide (H₂O₂). The remaining amount of hydrogen peroxide is quantified via an enzyme-linked color reaction measured spectrophotometrically at 520 nm, which minimizes interference compared to UV-based methods.
The method is optimized for reproducibility and reduced manual intervention by accounting for variations in substrate concentration in the calculation. The assay is suitable for laboratories that require a robust and standardized method for measuring enzyme activity in complex matrices.
Features
- Colorimetric method (520 nm) with reduced interference
- Quick analysis (~20 minutes)
- Hög reproducerbarhet (<3 % variation)
- Flexible substrate handling (reduces manual handling)
- Standards and calculation tools included
More information
| Parameter | Katalas |
|---|---|
| Result type | Quantitative |
| Analysis time | about 20 minutes |
| Number | |
| Storage temperature | 2–8°C |
| Sustainability | At least 24 months (lot-specific) |
| Other specifications | Detection method: Absorbance |
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