Enzymatic Assays and Their Applications: Discover Megazyme Tests

Megazyme Web

On our website, we are proud to present our extensive range of NeogenMegazyme, which utilize enzymatic assay methods to provide accurate and reliable analytical results across a wide range of applications. 

Enzymatic assays have revolutionized many industries by providing highly accurate and specific measurements of biochemical processes. Here, we explain how these tests work and why they are so important for modern research and industry.

What are enzymatic assays?

Enzymatic assays are techniques that utilize the ability of enzymes to catalyze specific chemical reactions. These assays are used to measure the concentration of substrates, enzymes, or products in a sample solution. The techniques are highly reliable and can be adapted for various types of measurements, ranging from spectrophotometry to fluorometry and chromatography. 

Applications of enzymatic assays

Enzymatic assays have a wide range of applications. They are used in everything from food analysis and medical diagnostics to biotechnology research and drug development. In the food industry, they are used to measure nutritional content and contaminants, while in medicine they can help detect enzyme defects and diseases.

  • Food analysis: Enzymes are used to quantify nutrients such as sugars, fats, and proteins, as well as to identify contaminants that may affect product quality and safety.
  • Drug development: Enzymatic assays are critical in drug development, where they are used to test how potential drugs interact with specific enzymes, which is essential for understanding the drug’s efficacy and safety.
  • Biotechnology: In biotechnology, enzymes are used to catalyze reactions that are essential for the production of biofuels, bioplastics, and other bio-based products.

The Benefits of Megazyme Tests

Megazyme offer several advantages that make them the best choice for your enzymatic analysis needs:

  • High precision: Our tests are designed to deliver accurate and reliable results every time, which is essential for ensuring quality and efficiency in your business.
  • Automation and Efficiency: With our advanced analysis tools, you can automate the entire testing process—from sample preparation to data analysis—saving time and reducing the risk of errors.
  • Flexibility: Megazyme can be customized to meet specific needs across various industries, making them a versatile tool for a wide range of applications.
The method principle for most Megazyme is shown below, followed by a list of available test kits.To use the kits, you will need aUV spectrophotometer and a standard laboratory equipped witha balance, vortex mixer, bench-top centrifuge, water bath, and heating bath. There are over 150 test methods to choose from.In our online store, we have listed only the most common kits, but we are working on adding Megazyme entire Megazyme. 
Enzymatic Assays and Their Applications: Discover Megazyme Tests
List of Megazyme :
ArticleTest kitNumber
K-ACHYDAcetaldehyde Assay Kit50 assays (manual) / 500 assays (microplate) / 500 assays (automated)
K-ACETAcetic Acid Assay Kit (ACS Manual Format)53 assays per kit
K-ACETAFAcetic Acid Assay Kit (ACS Analysis Format)456 assays (0.31 mL)
K-ACETAKAcetic Acid Assay Kit (Acetate Kinase Assay Format)550 assays (0.31 mL)
K-ACETGKAcetic Acid GK Assay Kit (Assay Format)500 assays (0.22 mL)
K-ACETRMAcetic Acid Assay Kit (Acetate Kinase Manual Format)72 assays (manual) / 720 assays (microplate)
K-AMIARAmmonia Assay Kit (Rapid)96 assays (manual) / 960 assays (microplate) / 960 assays (automated)
K-CERAα-Amylase Assay Kit (Ceralpha Method)100 assays per kit
K-AMYLSDα-Amylase SD Assay Kit160 assays (manual) / 640 assays (automated)
R-CAAR4α-Amylase Reagent (Ceralpha)200 assays per kit (4 vials)
R-AMHR4α-Amylase HR Reagent200 assays per kit (4 vials)
K-BETA3β-Amylase Assay Kit (Betamyl-3)100 assays per kit
R-BAMR3β-Amylase Assay Reagent (Betamyl-3)200 assays per kit (4 vials)
K-AMYLAmylose/Amylopectin Assay Kit100 assays per kit
R-AMGR3Amyloglucosidase Assay Reagent200 assays per kit (4 vials)
K-ARABArabinan Assay Kit100 assays per kit
K-ARGAL-Arabinose/D-Galactose Assay Kit115 assays (manual) / 1,150 assays (microplate) / 1,150 assays (automated)
K-LARGEL-Arginine/Urea/Ammonia Assay Kit50 assays per kit
K-ASCOAscorbic Acid Assay Kit (L-Ascorbate)40 assays (manual) / 400 assays (microplate) / 400 assays (automated)
K-ASNAML-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)100 assays (50 of each) per kit
K-ASPTMAspartame Assay Kit50 assays (manual) / 500 assays (microplate) / 500 assays (automated)
K-AVCHOAvailable Carbohydrates Assay Kit100 assays per kit
K-ACHDFAvailable Carbohydrates/Dietary Fiber Assay Kit100 assays of each component
K-CARREZCarrez Clarification Kit40–1,000 assays per kit (depending on the application)
K-CATALCatalase Assay Kit100 / 200 assays per kit
K-Cell G5-4VCellulase Assay Kit (CellG5 Method)Variations
K-CellG3Cellulase Assay Kit (CellG3 Method)180 / 360 assays per kit
K-CITRCitric Acid Assay Kit72 assays (manual) / 720 assays (microplate) / 840 assays (automated)
K-ETOHEthanol Assay Kit60 assays (manual) / 600 assays (microplate) / 600 assays (automated)
K-ETOHLQREthanol Assay Kit (Liquid Ready)60 assays (manual) / 600 assays (microplate) / 600 assays (automated)
K-TDFR-200ATotal Dietary Fiber Assay KitVariations
K-INTDFIntegrated Total Dietary Fiber Assay Kit100 assays per kit
K-RINTDFRapid Integrated Total Dietary Fiber Assay Kit100 assays per kit
K-TDFCTotal Dietary Fiber ControlsContains 6 controls: Use with K-TDFR, K-INTDF, or K-RINTDF
K-FRHYDFormaldehyde Assay Kit50 assays (manual) / 500 assays (microplate) / 500 assays (automated)
K-FORMFormic Acid Assay Kit25 assays (manual) / 250 assays (microplate) / 220 assays (automated)
K-FRUCHKFructan HK Assay Kit50 assays per kit
K-FRUCFructan Assay Kit100 assays per kit
K-FRUGLD-Fructose/D-Glucose Assay Kit110 assays (manual) / 1,100 assays (microplate) / 1,100 assays (automated)
K-FRGLQRD-Fructose/D-Glucose Assay Kit (Liquid Ready)1,100 assays (microplate) / 1,100 assays (automated assays)
K-FUCOSEL-Fucose Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,020 assays (automated)
K-GALMGalactomannan Assay Kit100 assays per kit
K-BGLUβ-Glucan Assay Kit (Mixed Linkage)100 assays per kit
K-EBHLGEnzymatic Yeast β-Glucan Assay Kit50 assays per kit
K-YBGLβ-Glucan Assay Kit (Yeast and Mushroom)100 assays per kit
K-MBG4Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)100 assays (manual) / 400 assays (automated)
K-MBGLβ-Glucanase Assay Kit (Malt and Microbial)100 assays per kit
K-GLUMGlucomannan Assay Kit50 assays per kit
K-GATED-Gluconic Acid/D-Glucono-δ-lactone Assay Kit60 assays (manual) / 600 assays (microplate) / 600 assays (automated)
K-GAMINED-Glucosamine Assay Kit50 assays (manual) / 500 assays (microplate) / 500 assays (automated)
K-GLUCD-Glucose Assay Kit (GOPOD Format)660 assays per kit
R-GLC4Glucose Determination Reagent1,300 assays per kit (4 vials)
K-GLUHK-220AD-Glucose HK Assay KitVariations
K-MRGLUCD-Glucose Assay Kit (Megaplex Red)1,000 assays (microplate) / 100 assays (manual) / 400 assays (automated)
K-GLOXGlucose Oxidase Assay Kit200 assays (manual) / 2,000 assays (microplate) / 1,960 assays (automated)
K-URONICD-Glucuronic/D-Galacturonic Acid Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,000 assays (automated)
K-AGLUAα-Glucuronidase Assay Kit50 assays (manual) / 200 assays (microplate)
K-GEUX3Glucuronoyl Esterase Assay Kit100 assays per kit
K-GLUTL-Glutamic Acid Assay Kit60 assays (manual) / 600 assays (microplate) / 700 assays (automated)
K-GLNAML-Glutamine/Ammonia Assay Kit (Rapid)50 assays each (manual) / 500 assays each (microplate)
K-GCROLGlycerol Assay Kit70 assays (manual) / 700 assays (microplate)
K-GCROLGKGlycerol GK Assay Kit70 assays (manual) / 700 assays (microplate) / 600 assays (automated)
K-HISTAHistamine Assay Kit100 assays per kit
K-MRH2O2Hydrogen Peroxide Assay Kit (Megaplex Red)1,000 assays (microplate) / 100 assays (manual) / 400 assays (automated)
K-HDBAD-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit60 assays (manual) / 600 assays (microplate) / 740 assays (automated)
K-INOSLMyo-Inositol Assay Kit50 assays per kit
K-ISOCD-Isocitric Acid Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,000 assays (automated)
K-DATED-Lactic Acid (D-Lactate) (Rapid) Assay Kit50 assays (manual) / 500 assays (microplate) / 450 assays (automated)
K-DLATED-/L-Lactic Acid (D-/L-Lactate) (Rapid) Assay Kit100 assays (50 of each) per kit
K-LATEL-Lactic Acid (L-Lactate) Assay Kit50 assays (manual) / 500 assays (microplate) / 450 assays (automated)
K-LOLACLactose Assay Kit – Sequential/High Sensitivity65 assays per kit
K-LACGARLactose/Galactose Assay Kit (Rapid)115 assays per kit
K-LACSULactose/Sucrose/D-Glucose Assay Kit100 assays per kit
K-LACTULLactulose Assay Kit50 assays per kit
K-DMALD-Malic Acid Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,100 assays (automated)
K-LMAL-116AL-Malic Acid Assay Kit (Manual Format)Variations
K-LMALAFL-Malic Acid Assay Kit (Assay Format)1,116 assays (0.22 mL)
K-LMALQRL-Malic Acid Assay Kit (Liquid Ready)1,100 assays (microplate) / 1,100 assays (automated assays)
K-LMALMQL-Malic Acid Assay Kit (MegaQuant™ Format)60 assays per kit
K-MALTAMalt Amylase Assay Kit100 assays (50 of each) per kit
K-MASUGMaltose/Sucrose/D-Glucose Assay Kit100 assays (34 of each) per kit
K-MANOLD-Mannitol Assay Kit60 assays (manual) / 600 assays (microplate) / 580 assays (automated)
K-MANGLD-Mannose/D-Fructose/D-Glucose Assay Kit55 assays per kit
K-PECIDPectin Identification Assay Kit500 assays per kit
K-PullG6Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)100/200 assays per kit
K-PHYTASEPhytase Assay Kit100 assays per kit
K-PHYTPhytic Acid Assay Kit50 assays per kit
K-PANOPAPrimary Amino Nitrogen Assay Kit (PANOPA)100 assays (manual) / 1,000 assays (microplate) / 1,100 assays (automated)
K-PDCAASProtein Digestibility Assay Kit50 assays per kit
K-PHOSPhosphate Assay Kit100 assays (manual) / 400 assays (automated)
K-PYRUVPyruvic Acid Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,000 assays (automated)
K-RAFGARaffinose/D-Galactose Assay Kit120 assays per kit
K-RAFGLRaffinose/Sucrose/D-Glucose Assay Kit120 assays per kit
K-RHAMNOSEL-Rhamnose Assay Kit50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (automated)
K-SORBD-Sorbitol/Xylitol Assay Kit58 assays (manual) / 580 assays (microplate) / 700 assays (automated)
K-DSTRSDigestible and Resistant Starch Assay Kit40 assays per kit
K-SDAMStarch Damage Assay Kit200 assays per kit
K-RAPRSResistant Starch Assay Kit (Rapid)100 assays per kit
K-RSTARResistant Starch Assay Kit100 assays per kit
K-RSTCLResistant Starch Control Flours5 controls per kit: For use with K-RSTAR
K-TSTA-100ATotal Starch Assay Kit (AA/AMG)Variations
K-TSHKTotal Starch HK Assay Kit100 assays per kit
K-TSCKTotal Starch Control Flours6 controls per kit: For use with K-TSTA or K-TSHK
K-SUCCSuccinic Acid Assay Kit20 assays (manual) / 200 assays (microplate) / 270 assays (automated)
K-SUFRGSucrose/D-Fructose/D-Glucose Assay Kit300 assays (100 of each) per kit
K-SUCGLSucrose/D-Glucose Assay Kit250 assays per kit
K-FSULPHFree Sulfite Assay Kit (Liquid Ready)80 assays (manual) / 800 assays (microplate) / 800 assays (automated)
K-TSULPHTotal Sulfite Assay Kit (Liquid Ready)80 assays (manual) / 800 assays (microplate) / 800 assays (automated)
K-ETSULPHTotal Sulfite Assay Kit (Enzymatic)50 assays (manual) / 500 assays (microplate) / 588 assays (automated)
K-SULPHTotal and Free Sulfite Assay Kit (Liquid Ready)40 assays each (manual) / 400 assays each (microplate) / 400 assays each (automated analyzer)
K-TARTTartaric Acid Assay Kit (Liquid Ready)200 assays (manual) / 2,000 assays (microplate) / 2,000 assays (automated)
K-TREHTrehalose Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,100 assays (automated)
K-URAMRUrea/Ammonia Assay Kit (Rapid)100 assays (50 of each) per kit
K-XylX6-2VXylanase Assay Kit (XylX6 Method)Variations
K-AZOWAXXylanase Assay Kit (Azo-Wax)200 assays per kit
K-XYLSXylanase Assay Kit (Xylazyme AX)200 assays per kit
K-XYLOSED-Xylose Assay Kit100 assays (manual) / 1,000 assays (microplate) / 1,300 assays (automated)

If you are looking for specific enzymes for your own research or analytical method, you can order individual enzymes. Download the catalog listing of available Megazyme .

Summary

Enzymatic assays offer a powerful and precise method for analyzing and understanding complex biochemical processes. By using Megazyme, you can ensure that you obtain the most reliable and accurate results, helping you make informed decisions and improve the quality of your products and services. Explore our range of Megazyme enzymatic assays today and discover how they can revolutionize your work!

Please feel free to read Megazyme’s product sheet for nutritional analysis and food analysis

For more information about our products and how they can benefit your business, please feel free tocontact us directly.

Shopping Cart
Scroll to the top